TSSK2 (Human) Recombinant Protein
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Specification
Product Description
Human TSSK2 (Q96PF2, 1 a.a. - 358 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
68
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
86 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000.
Substrate : GS peptide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TSSK2
Entrez GeneID
23617Protein Accession#
Q96PF2Gene Name
TSSK2
Gene Alias
DGS-G, FLJ38613, SPOGA2, STK22B
Gene Description
testis-specific serine kinase 2
Omim ID
610710Gene Ontology
HyperlinkGene Summary
TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM
Other Designations
serine/threonine kinase 22B (spermiogenesis associated)|spermiogenesis associated 2|testis specific serine threonine kinase 2
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