MYLK2 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human MYLK2 (NP_149109.1, 1 a.a. - 596 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
93
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
67 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000.
Substrate : MLCtide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MYLK2
Entrez GeneID
85366Protein Accession#
NP_149109.1Gene Name
MYLK2
Gene Alias
KMLC, MLCK, MLCK2, skMLCK
Gene Description
myosin light chain kinase 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. [provided by RefSeq
Other Designations
OTTHUMP00000030560|OTTHUMP00000030561|OTTHUMP00000030562|myosin light chain kinase 2, skeletal muscle|skeletal muscle myosin light chain kinase|skeletal myosin light chain kinase
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