PLK2 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PLK2 (Q9NYY3, 1 a.a. - 685 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
105
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
81 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).Substrate : CHK2 peptide. ATP: 100 µM.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PLK2
Entrez GeneID
10769Protein Accession#
Q9NYY3Gene Name
PLK2
Gene Alias
SNK
Gene Description
polo-like kinase 2 (Drosophila)
Omim ID
607023Gene Ontology
HyperlinkGene Summary
Serum-inducible kinase is a member of the 'polo' family of serine/threonine protein kinases that have a role in normal cell division.[supplied by OMIM
Other Designations
polo-like kinase 2|serum-inducible kinase
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