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Last updated: 2017/12/10

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PIM1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5793
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  • Specification
  • Product Description:
  • Human PIM1 (NP_002639.1, 1 a.a. - 313 a.a. ) full-length recombinant protein with His tag expressed in Baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 39
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Ni-NTA affinity chromatography
  • Purity:
  • 82 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000.
    Substrate : S6K2 peptide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5793
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5292
  • Gene Name:
  • PIM1
  • Gene Alias:
  • PIM
  • Gene Description:
  • pim-1 oncogene
  • Gene Summary:
  • The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000018046,Oncogene PIM1,non-specific serine/threonine protein kinase,pim-1 kinase 44 kDa isoform,pim-1 oncogene (proviral integration site 1)
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