NUAK2 (Human) Recombinant Protein
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Specification
Product Description
Human NUAK2 (NP_112214.1, 1 a.a - 628 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
98
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
74 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000.
Substrate : CHKtide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — NUAK2
Entrez GeneID
81788Protein Accession#
NP_112214.1Gene Name
NUAK2
Gene Alias
DKFZp434J037, DKFZp686F01113, FLJ90349, SNARK
Gene Description
NUAK family, SNF1-like kinase, 2
Omim ID
608131Gene Ontology
HyperlinkGene Summary
SNF1-like kinase
Other Designations
OTTHUMP00000035319|SNF1/AMP activated protein kinase
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