Human MELK (NP_055606.1, 1 a.a. - 493 a.a.) partial recombinant protein with GST tag expressed in Escherichia coli
Theoretical MW (kDa):
Escherichia coli expression system
Glutathione sepharose chromatography
62 % by SDS-PAGE/CBB staining
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000. Substrate : GS peptide. ATP: 100 µM.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.