MELK (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human MELK (NP_055606.1, 1 a.a. - 493 a.a.) partial recombinant protein with GST tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
84
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Glutathione sepharose chromatography
Purity
62 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : GS peptide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MELK
Entrez GeneID
9833Protein Accession#
NP_055606.1Gene Name
MELK
Gene Alias
HPK38, KIAA0175
Gene Description
maternal embryonic leucine zipper kinase
Omim ID
607025Gene Ontology
HyperlinkOther Designations
OTTHUMP00000046113|pEg3 kinase
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