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Last updated: 2016/12/11

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MELK (Human) Recombinant ProteinBioActive

  • Catalog # : P5785
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  • Specification
  • Product Description:
  • Human MELK (NP_055606.1, 1 a.a. - 493 a.a.) partial recombinant protein with GST tag expressed in Escherichia coli
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 84
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 62 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : GS peptide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5785
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 9833
  • Gene Name:
  • MELK
  • Gene Alias:
  • HPK38,KIAA0175
  • Gene Description:
  • maternal embryonic leucine zipper kinase
  • Other Designations:
  • OTTHUMP00000046113,pEg3 kinase
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