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AURKB/INCENP (Human) Recombinant ProteinBioActive

  • Catalog # : P5781
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  • Specification
  • Product Description:
  • Human AURKB (Q96GD4, 1 a.a. - 344 a.a.) full length recombinant protein with GST tag co-expressed with His-tagged INCENP (AAU04398.1, 803 a.a. - 918 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
  • Theoretical MW (kDa):
  • 66
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 86 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : Kemptide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5781
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 9212
  • Gene Name:
  • AURKB
  • Gene Alias:
  • AIK2,AIM-1,AIM1,ARK2,AurB,IPL1,STK12,STK5
  • Gene Description:
  • aurora kinase B
  • Gene Summary:
  • Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM
  • Other Designations:
  • aurora-1,aurora-B,serine/threonine kinase 12
  • Gene Information
  • Entrez GeneID:
  • 3619
  • Gene Name:
  • INCENP
  • Gene Alias:
  • FLJ31633,MGC111393
  • Gene Description:
  • inner centromere protein antigens 135/155kDa
  • Gene Summary:
  • In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM
  • Other Designations:
  • binds and activates aurora-B and -C in vivo and in vitro,chromosomal passenger protein,inner centromere protein INCENP,inner centromere protein antigens (135kD, 155kD)
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