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PAK4 (Human) Recombinant ProteinBioActive

  • Catalog # : P5779
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  • Specification
  • Product Description:
  • Human PAK4 (NP_005875.1, 1 a.a. - 591 a.a.) full length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 91
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 99 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : SGKtide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5779
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • PAK4
  • Gene Alias:
  • -
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 4
  • Gene Summary:
  • PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • p21(CDKN1A)-activated kinase 4,p21-activated kinase 4,protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
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