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MAPKAPK5 (Human) Recombinant ProteinBioActive

  • Catalog # : P5777
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  • Specification
  • Product Description:
  • Human MAPKAPK5 (NP_003659.2, 1 a.a. - 471 a.a.) full length recombinant protein with GST tag co-expressed with His-tagged MAPK11 (NP_002742.3, 1 a.a. - 364 a.a.) full length recombinant protein and His-tagged MAP2K6 (NP_002749, 1 a.a. - 334 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
  • Theoretical MW (kDa):
  • 81
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 90 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : GS peptide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5777
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 8550
  • Gene Name:
  • MAPKAPK5
  • Gene Alias:
  • PRAK
  • Gene Description:
  • mitogen-activated protein kinase-activated protein kinase 5
  • Gene Summary:
  • The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • p38-regulated/activated protein kinase
  • Interactome
  • Interactome
  • Gene Pathway
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