Human MAPK12 (NP_002960.2, 1 a.a. - 367 a.a.) full length recombinant protein with GST tag expressed in Escherichia coli.
Theoretical MW (kDa):
Escherichia coli expression system
Glutathione sepharose chromatography
97 % by SDS-PAGE/CBB staining
The activity was determined by IMAP™assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAP™technology (fluorescence polarization). Substrate : Erktide. ATP: 3 µM.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq
mitogen-activated protein kinase 3,stress-activated protein kinase 3