MAPK12 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human MAPK12 (NP_002960.2, 1 a.a. - 367 a.a.) full length recombinant protein with GST tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
69
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Glutathione sepharose chromatography
Purity
97 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAP™assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAP™technology (fluorescence polarization).
Substrate : Erktide. ATP: 3 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MAPK12
Entrez GeneID
6300Protein Accession#
NP_002960.2Gene Name
MAPK12
Gene Alias
ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3
Gene Description
mitogen-activated protein kinase 12
Omim ID
602399Gene Ontology
HyperlinkGene Summary
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq
Other Designations
mitogen-activated protein kinase 3|stress-activated protein kinase 3
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Interactome
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Pathway
- Amyotrophic lateral sclerosis (ALS)
- Epithelial cell signaling in Helicobacter pylori infection
- Fc epsilon RI signaling pathway
- GnRH signaling pathway
- Leukocyte transendothelial migration
- MAPK signaling pathway
- Neurotrophin signaling pathway
- T cell receptor signaling pathway
- Toll-like receptor signaling pathway
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