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MAPK11 (Human) Recombinant ProteinBioActive

  • Catalog # : P5773
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  • Specification
  • Product Description:
  • Human MAPK11 (NP_002742.3, 1 a.a. - 364 a.a.) full length recombinant protein with GST tag expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 69
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 81 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : Modified Erktide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5773
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5600
  • Gene Name:
  • MAPK11
  • Gene Alias:
  • P38B,P38BETA2,PRKM11,SAPK2,SAPK2B,p38-2,p38Beta
  • Gene Description:
  • mitogen-activated protein kinase 11
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000196655,mitogen-activated protein kinase p38 beta,mitogen-activated protein kinase p38-2,stress-activated protein kinase-2,stress-activated protein kinase-2b
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