SRPK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human SRPK1 (CAI20544.1, 1 a.a. - 654 a.a.) full length recombinant protein with GST tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
100
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Glutathione sepharose chromatography
Purity
39 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAP™assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAP™technology (fluorescence polarization).
Substrate : SRPKtide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SRPK1
Entrez GeneID
6732Protein Accession#
CAI20544.1Gene Name
SRPK1
Gene Alias
SFRSK1
Gene Description
SFRS protein kinase 1
Omim ID
601939Gene Ontology
HyperlinkGene Summary
This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined. [provided by RefSeq
Other Designations
SR protein kinase 1
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Interactome
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Disease
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