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KIAA1804 (Human) Recombinant ProteinBioActive

  • Catalog # : P5764
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  • Specification
  • Product Description:
  • Human KIAA1804 (NP_115811.2, 114 a.a. - 420 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 61
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 56 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
    Substrate : MAP2K7 [inactive mutant]. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5764
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • KIAA1804
  • Gene Alias:
  • MLK4,RP5-862P8.2,dJ862P8.3
  • Gene Description:
  • mixed lineage kinase 4
  • Other Designations:
  • OTTHUMP00000036006
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