KIAA1804 (Human) Recombinant Protein
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Specification
Product Description
Human KIAA1804 (NP_115811.2, 114 a.a. - 420 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
61
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
56 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
Substrate : MAP2K7 [inactive mutant]. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — KIAA1804
Entrez GeneID
84451Protein Accession#
NP_115811.2Gene Name
KIAA1804
Gene Alias
MLK4, RP5-862P8.2, dJ862P8.3
Gene Description
mixed lineage kinase 4
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000036006
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Interactome
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Disease
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