MKNK2 (T379D) (Human) Recombinant Protein
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Specification
Product Description
Human MKNK2 (NP_951009.1, 1 a.a. - 465 a.a.) T379D mutant full length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
79
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
91 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : RS peptide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MKNK2
Entrez GeneID
2872Protein Accession#
NP_951009.1Gene Name
MKNK2
Gene Alias
GPRK7, MNK2
Gene Description
MAP kinase interacting serine/threonine kinase 2
Omim ID
605069Gene Ontology
HyperlinkOther Designations
G protein-coupled receptor kinase 7|MAP kinase-interacting serine/threonine kinase 2|Putative map kinase interacting kinase
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Interactome
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Pathway
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Disease
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