Product Browser

Last updated: 2017/1/15

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

SGK3 (S486D) (Human) Recombinant ProteinBioActive

  • Catalog # : P5751
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human SGK3 (NP_037389.4, 119 a.a. - 496 a.a.) S486D mutant partial recombinant protein with GST tag co-expressed with His-tagged PDPK1 (NP_002604.1, 1 a.a. - 556 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
  • Theoretical MW (kDa):
  • 70
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 95 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : SGKtide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5751
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • SGK3
  • Gene Alias:
  • CISK,DKFZp781N0293,SGK2,SGKL
  • Gene Description:
  • serum/glucocorticoid regulated kinase family, member 3
  • Gene Summary:
  • This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • cytokine-independent survival kinase,serum/glucocorticoid regulated kinase 3,serum/glucocorticoid regulated kinase-like
  • RSS
  • YouTube
  • Linkedin
  • Facebook