SGK3 (S486D) (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human SGK3 (NP_037389.4, 119 a.a. - 496 a.a.) S486D mutant partial recombinant protein with GST tag co-expressed with His-tagged PDPK1 (NP_002604.1, 1 a.a. - 556 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
Theoretical MW (kDa)
70
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
95 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : SGKtide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SGK3
Entrez GeneID
23678Protein Accession#
NP_037389.4Gene Name
SGK3
Gene Alias
CISK, DKFZp781N0293, SGK2, SGKL
Gene Description
serum/glucocorticoid regulated kinase family, member 3
Omim ID
607591Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
cytokine-independent survival kinase|serum/glucocorticoid regulated kinase 3|serum/glucocorticoid regulated kinase-like
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