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SGK2 (S356D) (Human) Recombinant ProteinBioActive

  • Catalog # : P5749
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  • Specification
  • Product Description:
  • Human SGK2 (NP_733794.1, 1 a.a. - 367 a.a.) S356D mutant full length recombinant protein with GST tag co-expressed with His-tagged PDPK1 (NP_002604.1, 1 a.a. - 556 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
  • Theoretical MW (kDa):
  • 68
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 97 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : SGKtide. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5749
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • SGK2
  • Gene Alias:
  • H-SGK2,dJ138B7.2
  • Gene Description:
  • serum/glucocorticoid regulated kinase 2
  • Gene Summary:
  • This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Two alternate transcripts encoding two different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000031702,OTTHUMP00000031703,OTTHUMP00000031705
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