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MAP2K1 (P124L) (Human) Recombinant ProteinBioActive

  • Catalog # : P5746
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  • Specification
  • Product Description:
  • Human MAP2K1 (NP_002746.1, 1 a.a. - 393 a.a.) P124L mutant full length protein with GST tag co-expressed with His-tagged RAF1 (NP_002871.1, 306 a.a. - 648 a.a.) Y340D/Y341D mutant full length recombinant protein in Baculovirus infected Sf21 cells.
  • Theoretical MW (kDa):
  • 70
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Ni-NTA affinity chromatography
  • Purity:
  • 97 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
    Substrate : Erk2 inactive mutant. ATP: 100 µM.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5746
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 5604
  • Gene Name:
  • MAP2K1
  • Gene Alias:
  • Gene Description:
  • mitogen-activated protein kinase kinase 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
  • Other Designations:
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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