Human MAP2K1 (NP_002746.1, 1 a.a. - 393 a.a.) P124L mutant full length protein with GST tag co-expressed with His-tagged RAF1 (NP_002871.1, 306 a.a. - 648 a.a.) Y340D/Y341D mutant full length recombinant protein in Baculovirus infected Sf21 cells.
Theoretical MW (kDa):
Baculovirus infected insect cell (Sf21) expression system
Ni-NTA affinity chromatography
97 % by SDS-PAGE/CBB staining
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate : Erk2 inactive mutant. ATP: 100 µM.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)