TAOK3 (N47S) (Human) Recombinant Protein
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Specification
Product Description
Human TAOK3 (NP_057365.3, 1 a.a. - 898 a.a.) N47S mutant full length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
132
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
73 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAP™assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAP™technology (fluorescence polarization).
Substrate : PKCε peptide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TAOK3
Entrez GeneID
51347Protein Accession#
NP_057365.3Gene Name
TAOK3
Gene Alias
DKFZp666H245, DPK, FLJ31808, JIK, MAP3K18
Gene Description
TAO kinase 3
Gene Ontology
HyperlinkOther Designations
STE20-like kinase
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Interactome
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Pathway
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Disease
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