NUAK1 (Human) Recombinant Protein
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Specification
Product Description
Human NUAK1 (NP_775490.2, 1 a.a. - 661 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
102
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
90 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : CHKtide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — NUAK1
Entrez GeneID
9891Protein Accession#
NP_055655.1Gene Name
NUAK1
Gene Alias
ARK5, KIAA0537
Gene Description
NUAK family, SNF1-like kinase, 1
Omim ID
608130Gene Ontology
HyperlinkGene Summary
SNF1-like kinase
Other Designations
AMP-activated protein kinase family member 5|AMPK-related protein kinase 5
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Interactome
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Disease
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