NLK (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human NLK (NP_057315.2 121 a.a. - 515 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
72
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
62 % by SDS-PAGE/CBB staining.
Activity
The activity was determined by IMAP™assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAP™technology (fluorescence polarization).
Substrate : Multide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — NLK
Entrez GeneID
51701Protein Accession#
NP_057315.2Gene Name
NLK
Gene Alias
DKFZp761G1211, FLJ21033
Gene Description
nemo-like kinase
Omim ID
609476Gene Ontology
HyperlinkOther Designations
nemo like kinase
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Interactome
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Pathway
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Disease
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