NEK2 (Human) Recombinant Protein
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Specification
Product Description
Human NEK2 (NP_002488.1, 1 a.a. - 445 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
55
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
94 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : CDK7 peptide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — NEK2
Entrez GeneID
4751Protein Accession#
NP_002488.1Gene Name
NEK2
Gene Alias
HsPK21, NEK2A, NLK1
Gene Description
NIMA (never in mitosis gene a)-related kinase 2
Omim ID
604043Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035216
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Disease
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