MARK3 (Human) Recombinant Protein
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Specification
Product Description
Human MARK3 (NP_002367.4, 1 a.a. - 729 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
108
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
73 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : CHKtide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MARK3
Entrez GeneID
4140Protein Accession#
NP_002367.4Gene Name
MARK3
Gene Alias
CTAK1, KP78, PAR1A
Gene Description
MAP/microtubule affinity-regulating kinase 3
Omim ID
602678Gene Ontology
HyperlinkOther Designations
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Interactome
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