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JAK2 (JH1/JH2) (Human) Recombinant ProteinBioActive

  • Catalog # : P5723
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  • Specification
  • Product Description:
  • Human PRKX (NP_004963.1, 532 a.a. - 1132 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 97
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 81 % by SDS-PAGE/CBB staining.
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : Srctide. ATP: 100 µM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5723
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3717
  • Gene Name:
  • JAK2
  • Gene Alias:
  • JTK10
  • Gene Description:
  • Janus kinase 2 (a protein tyrosine kinase)
  • Gene Summary:
  • This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq
  • Other Designations:
  • Janus kinase 2,OTTHUMP00000021024,OTTHUMP00000043260,tyrosine-protein kinase JAK2
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