JAK2 (Human) Recombinant Protein
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Specification
Product Description
Human JAK2 (NP_004963.1, 826 a.a. - 1132 a.a.) partial recombinant protein with His tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
39
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Ni-NTA affinity chromatography and anion exchange chromatography
Purity
84 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Srctide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — JAK2
Entrez GeneID
3717Protein Accession#
NP_004963.1Gene Name
JAK2
Gene Alias
JTK10
Gene Description
Janus kinase 2 (a protein tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq
Other Designations
Janus kinase 2|OTTHUMP00000021024|OTTHUMP00000043260|tyrosine-protein kinase JAK2
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