IKBKE (Human) Recombinant Protein
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Specification
Product Description
Human IKBKE (NP_054721.1, 1 a.a. - 716 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
108
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
83 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : IkappaBalpha peptide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IKBKE
Entrez GeneID
9641Protein Accession#
NP_054721.1Gene Name
IKBKE
Gene Alias
IKK-i, IKKE, IKKI, KIAA0151, MGC125294, MGC125295, MGC125297
Gene Description
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Omim ID
605048Gene Ontology
HyperlinkGene Summary
O
Other Designations
IKK-related kinase epsilon|OTTHUMP00000034540|inducible IkappaB kinase
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