FER (Human) Recombinant Protein
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Specification
Product Description
Human FER (NP_005237.1, 1 a.a. - 822 a.a.) full-lenth recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
122
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
94 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Srctide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — FER
Entrez GeneID
2241Protein Accession#
NP_005237.1Gene Name
FER
Gene Alias
TYK3
Gene Description
fer (fps/fes related) tyrosine kinase
Omim ID
176942Gene Ontology
HyperlinkGene Summary
Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq
Other Designations
phosphoprotein NCP94
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Interactome
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Pathway
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Disease
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