Human EPHA3 (NP_005224.2, 1 a.a. - 464; 1 a.a. - 216 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Theoretical MW (kDa):
Baculovirus infected insect cell (Sf21) expression system
Glutathione sepharose chromatography
88 % by SDS-PAGE/CBB staining.
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000. Substrate : Blk/Lyntide. ATP: 100 µM.
Quality Control Testing:
Loading 1 ug protein in SDS-PAGE
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq