DAPK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human DAPK1 (NP_004929.1, 1 a.a. - 289 a.a.) partial recombinant protein with His tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
60
Form
Liquid
Preparation Method
Baculovirus expression system
Purification
Ni-NTA affinity chromatography
Purity
94 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : DAPK1tide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — DAPK1
Entrez GeneID
1612Protein Accession#
NP_004929.1Gene Name
DAPK1
Gene Alias
DAPK, DKFZp781I035
Gene Description
death-associated protein kinase 1
Omim ID
600831Gene Ontology
HyperlinkGene Summary
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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