Human MAP3K8 (NP_004929.1, 30 a.a. - 397 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Theoretical MW (kDa):
Baculovirus expression system
Glutathione sepharose chromatography
88 % by SDS-PAGE/CBB staining.
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate : MAP2K1 [inactive mutant]. ATP: 100 µM.
Quality Control Testing:
Loading 1 ug protein in SDS-PAGE
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq
Cancer Osaka thyroid oncogene,Ewing sarcoma transformant,OTTHUMP00000019392,OTTHUMP00000019393,cot (cancer Osaka thyroid) oncogene,proto-oncogene serine/threoine protein kinase,tumor progression locus-2