CSNK1A1 (Human) Recombinant Protein
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Specification
Product Description
Human CSNK1A1 (NP_001883.4, 1 a.a. - 337 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
66
Form
Liquid
Preparation Method
Baculovirus expression system
Purification
Glutathione sepharose chromatography and anion exchange chromatography
Purity
78 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : CHKtide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSNK1A1
Entrez GeneID
1452Protein Accession#
NP_001883.4Gene Name
CSNK1A1
Gene Alias
CK1, HLCDGP1, PRO2975
Gene Description
casein kinase 1, alpha 1
Omim ID
600505Gene Ontology
HyperlinkGene Summary
O
Other Designations
down-regulated in lung cancer
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Interactome
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Pathway
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Disease
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