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CDK5/CDK5R1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5691
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  • Specification
  • Product Description:
  • Human CDK5 (NP_004926.1, 1 a.a. - 292 a.a.) full-length recombinant protein with GST tag co-expressed with CDK5R1 (NP_003876.1, 99 a.a. - 307 a.a.) expressed in Baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 60
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system
  • Purification:
  • Glutathione sepharose chromatography and anion exchange chromatography
  • Purity:
  • 99 % by SDS-PAGE/CBB staining.
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : Modified Histone H1. ATP: 100 µM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5691
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1020
  • Protein Accession#:
  • NP_004926.1 (Gene ID : 1020); NP_003876.1 (Gene ID : 8851)
  • Gene Name:
  • CDK5
  • Gene Alias:
  • PSSALRE
  • Gene Description:
  • cyclin-dependent kinase 5
  • Other Designations:
  • protein kinase CDK5 splicing
  • Gene Information
  • Entrez GeneID:
  • 8851
  • Protein Accession#:
  • NP_004926.1 (Gene ID : 1020); NP_003876.1 (Gene ID : 8851)
  • Gene Name:
  • CDK5R1
  • Gene Alias:
  • CDK5P35,CDK5R,MGC33831,NCK5A,p23,p25,p35,p35nck5a
  • Gene Description:
  • cyclin-dependent kinase 5, regulatory subunit 1 (p35)
  • Gene Summary:
  • The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq
  • Other Designations:
  • CDK5 activator 1,TPKII regulatory subunit,cyclin-dependent kinase 5 activator 1,cyclin-dependent kinase 5, regulatory subunit 1,neuronal CDK5 activator,regulatory partner for CDK5 kinase,tau protein kinase II 23kDa subunit
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