CAMK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CAMK1 (NM_003656, 1 a.a. - 370 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
68
Form
Liquid
Preparation Method
Baculovirus expression system
Purification
Glutathione sepharose chromatography
Purity
79 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg (or Mn)/ATP, and Ca/Calmodulin. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : GS peptide. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CAMK1
Entrez GeneID
8536Protein Accession#
NM_003656Gene Name
CAMK1
Gene Alias
CAMKI, MGC120317, MGC120318
Gene Description
calcium/calmodulin-dependent protein kinase I
Omim ID
604998Gene Ontology
HyperlinkGene Summary
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq
Other Designations
OTTHUMP00000158527
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Disease
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