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AURKC (isoform 3) (Human) Recombinant ProteinBioActive

  • Catalog # : P5686
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  • Specification
  • Product Description:
  • Human AURKC (NP_002392.2, 1 a.a. - 275 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 59
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 80 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
    Substrate : Kemptide. ATP: 100 µM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5686
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 6795
  • Gene Name:
  • AURKC
  • Gene Alias:
  • AIE2,AIK3,AurC,STK13,aurora-C
  • Gene Description:
  • aurora kinase C
  • Gene Summary:
  • This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • aurora/IPL1-related kinase 3,aurora/IPL1/EG2 protein 2,serine/threonine kinase 13 (aurora/IPL1-like)
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