MAP2K7 (K149M) (Human) Recombinant Protein
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Specification
Product Description
Human MAP2K7 (NP_660186.1, 1 a.a. - 419 a.a.) K149M mutant full-length recombinant protein with GST tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
74
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Glutathione sepharose chromatography
Purity
55 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: JNK1. ATP: 10 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MAP2K7
Entrez GeneID
5609Protein Accession#
NP_660186.1Gene Name
MAP2K7
Gene Alias
Jnkk2, MAPKK7, MKK7, PRKMK7
Gene Description
mitogen-activated protein kinase kinase 7
Omim ID
603014Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq
Other Designations
JNK kinase 2|JNK-activating kinase 2|MAP kinase kinase 7|OTTHUMP00000174397|c-Jun N-terminal kinase kinase 2|dual specificity mitogen-activated protein kinase kinase 7
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