Product Browser

Last updated: 2017/5/28

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

PIK3CB/PIK3R1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5669
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human PIK3CB (NP_006210.1, 1 a.a. - 1070 a.a.) and PIK3R1 (NP_852664.1, 1 a.a. - 724 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 150
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 96 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Phosphatidylinositol. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5669
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5291
  • Protein Accession#:
  • NP_006210.1 (Gene ID : 5291);NP_852664.1 (Gene ID : 5295)
  • Gene Name:
  • PIK3CB
  • Gene Alias:
  • DKFZp779K1237,MGC133043,PI3K,PI3KCB,PI3Kbeta,PIK3C1,p110-BETA
  • Gene Description:
  • phosphoinositide-3-kinase, catalytic, beta polypeptide
  • Gene Summary:
  • Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM
  • Other Designations:
  • PI3-kinase p110 subunit beta,PtdIns-3-kinase p110,phosphatidylinositol 3-kinase, catalytic, beta polypeptide
  • Gene Information
  • Entrez GeneID:
  • 5295
  • Protein Accession#:
  • NP_006210.1 (Gene ID : 5291);NP_852664.1 (Gene ID : 5295)
  • Gene Name:
  • PIK3R1
  • Gene Alias:
  • GRB1,p85,p85-ALPHA
  • Gene Description:
  • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
  • Gene Summary:
  • Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha),phosphatidylinositol 3-kinase, regulatory, 1,phosphatidylinositol 3-kinase-associated p-85 alpha,phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha),phosphoinositide-3-ki
  • Interactome 1
  • Interactome
  • Interactome 2
  • Interactome
  • RSS
  • YouTube
  • Linkedin
  • Facebook