PIK3CA/PIK3R1 (Human) Recombinant Protein
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Specification
Product Description
Human PIK3CA (NP_006209.2, 1 a.a. - 1068 a.a.) full-length recombinant protein was coexpressed as N-terminal GST-fusion protein (151 kDa) with PIK3R1 (NP_852664.1, 1 a.a. - 724 a.a.) expressed in baculovirus expression system.
Host
insect
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
90% by SDS-PAGE/CBB staining
Activity
The activity was determined by ADP-Glo™ Assay. The enzyme was incubated with Lipid substrate and Mg(or Mn)/ATP. The phosphorylation was detected by the ADP-Glo™ Kinase Assay technology (luminescent ADP detection assay).
Substrate : PI(4,5)P2 and Phosphatidylserine
ATP : 100 uMQuality Control Testing
Loading 1 ug protein in SDS-PAGE/CBB staining.
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.01% Brij35, 1 mM DTT, 10% glycerol).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — PIK3CA
Entrez GeneID
5290Protein Accession#
NP_006209.2; NP_852664.1Gene Name
PIK3CA
Gene Alias
MGC142161, MGC142163, PI3K, p110-alpha
Gene Description
phosphoinositide-3-kinase, catalytic, alpha polypeptide
Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq
Other Designations
PI3-kinase p110 subunit alpha|PtdIns-3-kinase p110|phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha|phosphatidylinositol 3-kinase, catalytic, alpha polypeptide|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
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Gene Info — PIK3R1
Entrez GeneID
5295Protein Accession#
NP_006209.2; NP_852664.1Gene Name
PIK3R1
Gene Alias
GRB1, p85, p85-ALPHA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Omim ID
171833Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase, regulatory, 1|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)|phosphoinositide-3-ki
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