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PIK3CA/PIK3R1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5668
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  • Specification
  • Product Description:
  • Human PIK3CA (NP_006209.2, 1 a.a. - 1068 a.a.) and PIK3R1 (NP_852664.1, 1 a.a. - 724 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 151
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 96 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Phosphatidylinositol. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5668
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5290
  • Protein Accession#:
  • NP_006209.2 (Gene ID : 5290);NP_852664.1 (Gene ID : 5295)
  • Gene Name:
  • PIK3CA
  • Gene Alias:
  • MGC142161,MGC142163,PI3K,p110-alpha
  • Gene Description:
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • Gene Summary:
  • Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq
  • Other Designations:
  • PI3-kinase p110 subunit alpha,PtdIns-3-kinase p110,phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha,phosphatidylinositol 3-kinase, catalytic, alpha polypeptide,phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
  • Gene Information
  • Entrez GeneID:
  • 5295
  • Protein Accession#:
  • NP_006209.2 (Gene ID : 5290);NP_852664.1 (Gene ID : 5295)
  • Gene Name:
  • PIK3R1
  • Gene Alias:
  • GRB1,p85,p85-ALPHA
  • Gene Description:
  • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
  • Gene Summary:
  • Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha),phosphatidylinositol 3-kinase, regulatory, 1,phosphatidylinositol 3-kinase-associated p-85 alpha,phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha),phosphoinositide-3-ki
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