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WNK3 (Human) Recombinant ProteinBioActive

  • Catalog # : P5664
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  • Specification
  • Product Description:
  • Human WNK3 (NP_065973.2, 1 a.a. - 434 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 76
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 92 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: SPAKtide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5664
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • WNK3
  • Gene Alias:
  • KIAA1566,PRKWNK3
  • Gene Description:
  • WNK lysine deficient protein kinase 3
  • Gene Summary:
  • Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function (Holden et al., 2004 [PubMed 15194194]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000023369,protein kinase, lysine deficient 3
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