WNK2 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human WNK2 (NP_006639.3, 166 a.a. - 489 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
65
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography and anion exchange chromatography
Purity
89 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: SPAKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — WNK2
Entrez GeneID
65268Protein Accession#
NP_006639.3Gene Name
WNK2
Gene Alias
KIAA1760, NY-CO-43, P/OKcl.13, PRKWNK2, SDCCAG43
Gene Description
WNK lysine deficient protein kinase 2
Omim ID
606249Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine. [provided by RefSeq
Other Designations
mitogen-activated protein kinase kinase kinase|protein kinase, lysine deficient 2|serologically defined colon cancer antigen 43
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Interactome
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Disease
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