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WNK1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5662
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  • Specification
  • Product Description:
  • Human WNK1 (NP_061852.1, 1 a.a. - 491 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 81
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 79 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: SPAKtide. ATP : 200 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5662
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • WNK1
  • Gene Alias:
  • KDP,KIAA0344,PHA2C,PRKWNK1,PSK
  • Gene Description:
  • WNK lysine deficient protein kinase 1
  • Gene Summary:
  • The WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000115370,kinase deficient protein,prostate-derived sterile 20-like kinase,protein kinase, lysine deficient 1,serine/threonine-protein kinase WNK1
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