WNK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human WNK1 (NP_061852.1, 1 a.a. - 491 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
83 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay (MSA). The enzyme was incubated with fluorecence-labeled substrate and Mn/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. Substrate: SPAKtide. ATP : 200 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — WNK1
Entrez GeneID
65125Protein Accession#
NP_061852.1Gene Name
WNK1
Gene Alias
KDP, KIAA0344, PHA2C, PRKWNK1, PSK
Gene Description
WNK lysine deficient protein kinase 1
Gene Ontology
HyperlinkGene Summary
The WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM
Other Designations
OTTHUMP00000115370|kinase deficient protein|prostate-derived sterile 20-like kinase|protein kinase, lysine deficient 1|serine/threonine-protein kinase WNK1
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Interactome
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Disease
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