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VRK2 (Human) Recombinant ProteinBioActive

  • Catalog # : P5661
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  • Specification
  • Product Description:
  • Human VRK2 (NP_006287.2, 1 a.a. - 483 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 83
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 83 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by ELISA. The enzyme was incubated with biotinylated peptide in streptavidin-coated ELISA plate. Phosphorylation was detected by HRP-labeled anti-phospho antibody. Substrate: ATF2. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5661
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 7444
  • Gene Name:
  • VRK2
  • Gene Alias:
  • -
  • Gene Description:
  • vaccinia related kinase 2
  • Gene Summary:
  • This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • vaccinia virus B1R-related kinase 2,vaccinia-related kinase-2
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