ULK3 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human ULK3 (NP_001092906.1, 1 a.a. - 472 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
81
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
96 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: MBP. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — ULK3
Entrez GeneID
25989Protein Accession#
NP_001092906.1Gene Name
ULK3
Gene Alias
DKFZp434C131, FLJ90566
Gene Description
unc-51-like kinase 3 (C. elegans)
Gene Ontology
HyperlinkOther Designations
unc-51-like kinase 3
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Interactome
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Pathway
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Disease
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