TTBK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human TTBK1 (NP_115927.1, 1 a.a. - 343 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
66
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
91 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: GS peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TTBK1
Entrez GeneID
84630Protein Accession#
NP_115927.1Gene Name
TTBK1
Gene Alias
BDTK, KIAA1855, RP3-330M21.4
Gene Description
tau tubulin kinase 1
Gene Ontology
HyperlinkOther Designations
brain-derived tau kinase
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