TSSK1B (Human) Recombinant Protein
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Specification
Product Description
Human TSSK1B (NP_114417.1, 1 a.a. - 367 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
42
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
66 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: GS peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TSSK1B
Entrez GeneID
83942Protein Accession#
NP_114417.1Gene Name
TSSK1B
Gene Alias
FKSG81, SPOGA4, STK22D, TSSK1
Gene Description
testis-specific serine kinase 1B
Omim ID
610709Gene Ontology
HyperlinkGene Summary
TSSK1 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM
Other Designations
OTTHUMP00000159055|serine/threonine kinase 22D (spermiogenesis associated)|serine/threonine kinase FKSG81|spermiogenesis associated 4|testis-specific serine kinase 1
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