TAOK2 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human TAOK2 (NP_004774.1, 1 a.a. - 319 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
63
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
88 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: TAOKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TAOK2
Entrez GeneID
9344Protein Accession#
NP_004774.1Gene Name
TAOK2
Gene Alias
KIAA0881, MAP3K17, PSK, PSK1, TAO1, TAO2
Gene Description
TAO kinase 2
Gene Ontology
HyperlinkOther Designations
prostate derived STE20-like kinase PSK|thousand and one amino acid protein kinase
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Interactome
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Pathway
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Disease
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