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MYLK (Human) Recombinant ProteinBioActive

  • Catalog # : P5641
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  • Specification
  • Product Description:
  • Human MYLK (NP_444253.2, 1428 a.a. - 1771 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 67
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 97 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and Ca/Calmodulin. The phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: GS peptide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5641
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 4638
  • Gene Name:
  • MYLK
  • Gene Alias:
  • DKFZp686I10125,FLJ12216,KRP,MLCK,MLCK1,MLCK108,MLCK210,MSTP083,MYLK1,smMLCK
  • Gene Description:
  • myosin light chain kinase
  • Gene Summary:
  • This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000180642,OTTHUMP00000180643,myosin, light polypeptide kinase,smooth muscle myosin light chain kinase
  • Interactome
  • Interactome
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