SLK (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human SLK (NP_055535.1, 1 a.a. - 1152 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
160
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
68 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Moesin-derived peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SLK
Entrez GeneID
9748Protein Accession#
NP_055535.1Gene Name
SLK
Gene Alias
KIAA0204, LOSK, MGC133067, STK2, bA16H23.1, se20-9
Gene Description
STE20-like kinase (yeast)
Gene Ontology
HyperlinkGene Summary
yeast
Other Designations
CTCL tumor antigen se20-9|Long Ste20-like Kinase|OTTHUMP00000020426|OTTHUMP00000059183|SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase|SNF1 sucrose nonfermenting like kinase|Ste20-related serine/threonine k
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Interactome
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Disease
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