RPS6KA6 (Human) Recombinant Protein
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Specification
Product Description
Human RPS6KA6 (NP_055311.1, 1 a.a. - 745 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
111
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
69 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: S6K peptide(N-FL). ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — RPS6KA6
Entrez GeneID
27330Protein Accession#
NP_055311.1Gene Name
RPS6KA6
Gene Alias
RSK4
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 6
Omim ID
300303Gene Ontology
HyperlinkGene Summary
90kDa
Other Designations
OTTHUMP00000023618|bA326A14.2 (ribosomal protein S6 kinase, 90kD, polypeptide 6)|bA54F22.1.1 (ribosomal protein S6 kinase, 90kD, polypeptide 6)|ribosomal S6 kinase 4|ribosomal protein S6 kinase, 90kD, polypeptide 6
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Interactome
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Disease
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