SIK2 (Human) Recombinant Protein
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Specification
Product Description
Human SIK2 (NP_056006.1, 1 a.a. - 926 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
132
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
73 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: AMARA peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SIK2
Entrez GeneID
23235Protein Accession#
NP_056006.1Gene Name
SIK2
Gene Alias
DKFZp434K1115, KIAA0781, LOH11CR1I, QIK, SNF1LK2
Gene Description
salt-inducible kinase 2
Omim ID
608973Gene Ontology
HyperlinkOther Designations
SNF1-like kinase 2|salt-inducible serine/threonine kinase 2
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Interactome
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Disease
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