PKN2 (Human) Recombinant Protein
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Specification
Product Description
Human PKN2 (NP_006247.1, 1 a.a. - 984 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
139
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
91 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: GS peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PKN2
Entrez GeneID
5586Protein Accession#
NP_006247.1Gene Name
PKN2
Gene Alias
MGC150606, MGC71074, PAK2, PRK2, PRKCL2, PRO2042, Pak-2
Gene Description
protein kinase N2
Omim ID
602549Gene Ontology
HyperlinkOther Designations
OTTHUMP00000011760|cardiolipin-activated protein kinase Pak2|protein kinase C-like 2
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