Human PRKG1 (NP_006249.1, 1 a.a. - 686 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Theoretical MW (kDa):
Baculovirus infected insect cell (Sf21) expression system
Glutathione sepharose chromatography
80 % by SDS-PAGE/CBB staining
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and cGMP. The phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: PKAtide3. ATP: 100 uM.
Quality Control Testing:
Loading 1 ug protein in SDS-PAGE
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.